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- Monk Fruit (Siraitia grosvenorii)
- Chemistry
- Pharmacology
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Date:
07-15-2014 | HC# 061461-500
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Re: Review of the Chemistry and Pharmacology of Monk Fruit
Li
C, Lin L-M, Sui F, et al. Chemistry and pharmacology of Siraitia grosvenorii: a review.
Chin J Nat Med. February 2014;12(2):89-102.
Monk
fruit (Siraitia grosvenorii), a
member of the Cucurbitaceae family, is found throughout southern China and
Indonesia. Monk fruit is traditionally used to sweeten foods and to treat cold
symptoms. Studies report that monk fruit has antioxidant, antidiabetic, and
anticancer bioactivity. The main bioactive compounds are thought to be the mogrosides,
which are triterpenoid glycosides occurring in the fruit. Monk fruit is
contemporarily used as an expectorant and for diabetes treatment. This review
discusses the current bioactivity of monk fruit extracts and its constituents.
Phytochemical
investigations have found that cucurbitane triterpenoid glycosides are the
prevalent compounds in monk fruit. Of these, the mogrosides (some of them are
characterized by a sweet taste) are considered the primary bioactive compounds
in monk fruit. Commercial products containing mogroside-enriched extracts that
are reportedly up to 200 times sweeter than sucrose are available as natural
sweeteners on the market in the United States. Additionally, flavonoids have
been found in the aboveground parts of monk fruit, and anthraquinones,
alkaloids, simple phenols, sterols, and aliphatic acids have been found in
fruits or leaves. The presence of polysaccharides and vitamin C has also been
reported.
In
mice, the aqueous extract of monk fruit (25 or 50 g/kg) decreased cough caused
by ammonia or sulfur dioxide. The same effect was seen in vitro with mogroside
treatment with a minimum cough inhibitory concentration of 80 mg/kg. Monk fruit
aqueous extract and mogrosides (50-200 mg/kg) were reported to promote phlegm
expulsion in mice and rats. Also in rats, aqueous extract of monk fruit
elevated blood lymphocytes (25 or 50 g/kg) and modulated the activity of
phagocytes in mice, suggesting an immunostimulatory effect.
When
compared to the standard antioxidants butylated hydroxytoluene (BHT) and rutin,
aqueous, ethanol, ethyl acetate, and chloroform extracts of monk fruit stem were
found to have stronger antioxidant activity than BHT, but lesser activity than
rutin. In diabetic mice, supplementation with 0.5, 1.0, or 3.0 g/kg of monk
fruit powder or extract for 30 days significantly reduced fasting and
after-meal blood glucose. Additionally, mogroside extract at 150 or 300 mg/kg
given for 30 days decreased blood glucose concentrations in mice, as well as
the expression of interferon-gamma (IFN-γ) and tumor necrosis factor-alpha
(TNF-α), both markers of inflammation. Mogroside extract administered at 100,
300, and 500 mg/kg significantly lowered blood glucose, total cholesterol, and
triacylglycerol concentrations in diabetic mice; these dosages also elevated
high-density lipoprotein cholesterol concentrations as well as liver
antioxidant enzyme activity.
When
tested with mouse skin tumors, mogroside V and 11-oxo-mogroside V were found to
have inhibitory activity on peroxynitrite-induced carcinogenesis. Antibacterial
effects were observed with ethanol extracts of the leaf and stem. Extract
concentrations at 50 mg/ml inhibited the proliferation of Pseudomonas aeruginosa by 90.9% (leaf) and 76.7% (stem),
respectively. Extracts from various parts of monk fruit were found to be
antibacterial against Streptococcus
mutans. Aloe emodin was found to be the strongest inhibitor of the tested monk
fruit isolates. In mice, a tablet of monk fruit extract attenuated swelling in
the ears and paws, as well as alleviated pain. The anti-inflammatory effects
may be due to downregulation of inducible nitric oxide synthase (iNOS) and
cyclooxygenase-2 (COX-2).
An
ethanol extract of monk fruit significantly extended the time to exhaustion in
mice undergoing a swimming test; following this test, hemoglobin and
antioxidant enzyme activity (superoxide dismutase and glutathione peroxidase)
in the liver were elevated in mice consuming the monk fruit extract, as
compared to control animals. The flavonoid fraction of monk fruit leaf also
lengthened the time to exhaustion in rats in a swimming test.
Other
reported effects were the attenuation of histamine release by a monk fruit water
extract and the triterpene glycoside fraction at dosages of 300 and 1,000 µg/ml
in mice, and prevention of endothelial cell injury by the total leaf flavones.
In
studies addressing toxicity, the maximum tolerated oral dose in mice exceeded
100 g/kg. Rats on a diet of 0.04%, 0.2%, 1%, and 5% of monk fruit extract for
13 weeks showed no physiological changes. Dogs consuming mogrosides for four weeks
at 3 g/kg also displayed no changes in blood parameters or organ health. Thus,
this review concludes that monk fruit and mogrosides are safe to consume.
Overall,
monk fruit has been shown to have a range of bioactivity, including
expectorant, antidiabetic, and antibacterial activity, although the effects are
based on in vitro and animal studies.
One small study1 evaluating the effects of mogrosides on blood sugar
in human volunteers was not included. The mogrosides are the most studied of
the monk fruit compounds, although other classes of compounds such as
flavonoids and polysaccharides are also reported to be bioactive. It is surmised
that further study is necessary to elucidate mechanisms of action of monk fruit
bioactivity, and synergistic activity of mogrosides with additional compounds
should also be investigated. Ideally, monk fruit will be the subject of future
clinical trials.
—Amy
C. Keller, PhD
Reference
1Xu Q, Liang R-G, Su X-J,
Zhang J-Z, Xu L. Study on normal human body blood sugar and liver enzymes changes
affected by oral mogrosides intake. Food Science. 2007;28(6):315-317.
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